Somlyai Gábor: Let's Defeat Cancer! The biological effect of deuterium depletion (Budapest, 2001)
Book Two - To the researcher
7. Dd-water (110 ppm) induced apoptosis in mice with transplanted human PC-3 prostate tumours [(5, 6), Semmelweis Orvostudományi Egyetem/ Semmelweis Medical School (SOTE), Budapest], 8. Dd-water (90 ppm) induced apoptosis in interleukin-dependent A4 cell line in vitro [(5), Paterson Institute)]. 9. Dd-water (90 ppm) activated the membrane transport process of the seaweed Elodea Canadensis, causing an increase in pH in cells, and a decrease in the extracellular area [(2, 4 and 11), József Attila Tudományegyetem/ József Attila University of Life Science (JATE), Szeged, Hungary], 10. Dd-water (90 ppm), simultaneously with the pH-change, hyperpolarised the cell membrane in the seaweed Elodea Canadensis [(11), JATE], 11. Dd-water (20, 60, 110 ppm), during germination, inhibited the sprouting of 10 different plant species [(2, 4, and 8), JATE]. 12. In the case of rice, corn, wheat, and squash, a clear dose dependence was observed showing that the lower the D-concentration, the stronger the inhibition of sprouting [(2, 4, 8), JATE]. 13. Dd-water inhibited DNA synthesis in vitro, in MCF—7 (human breast), PC-3 (human prostate), and M14 (human melanoma) cell lines [(5), Oncotech Incorporation, Irvine, USA]. 14. Inhibition of DNA synthesis, in the case of MCF-7 (human breast), PC-3 (human prostate), and M14 (human melanoma) cell lines was higher and also lasted longer with cells synchronised in the phase G^G, in vitro [(5), Oncotech Incorporation]. 15. In the inhibition of DNA synthesis, a dose dependence was clearly evident: the lower the D-concentration of the culture medium, the stronger the inhibition [Oncotech Incorporation]. 16. The application of Dd-water (90 ppm) resulted in the total cure of a cat with lymphoid leucosis [(3), Alpha-Vet Veterinary Hospital, Székesfehérvár, Hungary]. 17. Dd-water (90 ppm) resulted in significant tumour regression and healing in dogs with breast, rectum, and testis cancer [(2, 4, 5, 6, and 7), Alpha- Vet Veterinary Hospital]. 18. Dd-water inhibited the proliferation of a HT-29 human colon tumour cell line in vitro but exerted a minimal effect only on healthy myometrial cells [SOTE], 19. The inhibitory effect of Dd-water was more expressed in the case of HT- 29 cell line, too, when cells synchronised in phase G0/G, were placed in a culture medium with Dd-water [SOTE]. 20. Inhibition of DNA synthesis was observed as a result of deuterium depletion in case of tumours of the ovary and breast from newly operated tumour patients in vitro [Oncotech Incorporation], 21. During experiments carried out with human tumours, the inhibitory effect of Dd-water doubled (increased to 40 percent) in the experimental system, 58